FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004048701

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048701
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1988981
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC564432.8377847752190695No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGC246731.2404844490721632No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT120460.6056367557055597No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG91450.4597831754048933No Hit
GTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACA80270.40357348813286803No Hit
CGTCAGATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCT76100.38260797865841856No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA75760.3808985606197344No Hit
GTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACA55750.2802942813430596No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACCTGTCTCTTATACA52800.26546256600741786No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA46290.23273223826673056No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCC45520.22886090917912236No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGC37070.18637684321770795No Hit
GATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTAT29280.14721105933138626No Hit
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT28380.1426861292289871TruSeq Adapter, Index 10 (95% over 21bp)
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGA24780.12458640881939044No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTA24520.12327920678980844No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACCTGTCTCTTATACACA24130.12131840374543547No Hit
GGACTCCTCCTTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC23930.1203128637226801No Hit
GTGTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACATCTCCGAGC21110.10613474940182938No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC20650.10382200734949203No Hit
CGTCAGATGTGTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACAT20150.10130815729260359No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA19970.10040317127212375No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)