FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004048702

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048702
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1976973
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC941324.761420616265371No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGG404622.0466642690618433No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG173340.8767949789906084No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA163880.8289440472884556No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGG91020.4604008248974569No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT83730.42352626970626306No Hit
GTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACA73500.37178049472602814No Hit
CGTCAGATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCT52720.2666703085980436No Hit
GTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACA48360.24461639081565606No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAG47960.24259309560626272No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACCTGTCTCTTATACA45690.23111089529295545No Hit
TTTGCAGATGATGGTGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC34850.17627959511839564No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCC34560.17481270609158547No Hit
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACAAG31230.15796877347338584No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTA29610.14977442787534276No Hit
CGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTC28660.14496910175303357No Hit
TCCTCAATGCTGTTGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGA27250.13783698613992199No Hit
GCGTAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGC27110.13712883281663432No Hit
GATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTAT25430.12863099293718225No Hit
TGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTC24440.1236233372939337No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACCTGTCTCTTATACACA23810.12043664733913917No Hit
TGGTGTCTGTGATGGTGCTGTCTCTTATACACATCTCCGAGCCCACGAGA23450.11861568165068516No Hit
TGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTC21300.10774046990019591No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA20270.10253048473600802No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)