FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004048705

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048705
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1021681
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC380783.7269950209507665No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT189971.8593866382951234No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAG140851.3786103490228359No Hit
GTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACA70950.6944437647367426No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCC64470.6310188796698775No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG61550.6024385302261664No Hit
CGTCAGATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCT58170.5693557969659806No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA47290.4628646319154413No Hit
GTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACA46620.45630681200883644No Hit
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT39880.39033710130657223RNA PCR Primer, Index 17 (95% over 21bp)
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACCTGTCTCTTATACA39800.38955407803414177No Hit
ACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCT26370.25810404617488236RNA PCR Primer, Index 17 (95% over 23bp)
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAG26050.25497195308516063No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGG23930.23422183636575408No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATGTCGTATGCCGT21890.21425474291877797No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACCTGTCTCTTATACACA21240.20789267883028067No Hit
GATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTAT20110.1968324751072008No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTT19110.18704468420182035No Hit
GGACTCCTCCTTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC17580.17206936411658824No Hit
CGTCAGATGTGTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACAT16200.15856221266716322No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTA15460.15131924739718172No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCTGT15420.1509277357609665No Hit
GTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCC14860.14544657285395343No Hit
GTGTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACATCTCCGAGC14480.14172721230990887No Hit
TCCTCAATGCTGTTGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGA13380.13096064231399038No Hit
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACAGG13200.12919883995102188No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATGTCGTATGCC13050.12773067131521482No Hit
CGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTC13030.12753491549710722No Hit
TCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAG12570.1230325316806322No Hit
GCGTAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGA12320.1205855839542871No Hit
GTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG12130.11872590368226482No Hit
ACTTAGAACTGGTACGGACAAGGGGAATCTGACTGTCTAATTAAAACATA12120.11862802577321101No Hit
GTGTATAAGAGACAGCCAGGTCTGAAGGCAGTCTCTTATACACATCTCCG11920.11667046759213492No Hit
TTTGCAGATGATGGTGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC11830.11578956641065069No Hit
GCGTCAGATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTC10950.1071763104139159No Hit
GTATAAGAGACAGTGTCCATGCATCCATAGCGTCTGTCTCTTATACACAT10540.1031633161427099No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)