FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004048707

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048707
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1437278
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC509803.5469825600892797No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT299942.0868614144236535No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTC139660.971697890039366No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCC125260.8715085042698767No Hit
CGTCAGATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCT121080.8424257520117889No Hit
GTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACA116870.8131342718666813No Hit
GTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACA83570.5814463172747374No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG64040.4455644628248675No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACCTGTCTCTTATACA59330.4127941845627638No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT55670.3873293823463519RNA PCR Primer, Index 27 (95% over 23bp)
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA55520.38628574291125306No Hit
GATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTAT39130.2722507406361191No Hit
ACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCT34790.24205477298059247RNA PCR Primer, Index 27 (96% over 25bp)
GTATCAACGCAGAGTACATGGGGTGGCGGCGACGACCCATTCGAACGTCT31230.21728573038757984No Hit
GTACATGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTT28670.19947428402855955No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGA28200.19620421379858316No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACCTGTCTCTTATACACA27650.19237753586988737No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTA26320.18312393287867762No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTC24380.16962619618473251No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCTGT23840.16586909421837667No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA22810.1587027700973646No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT21790.15160602193869244No Hit
GCGTCAGATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTC21370.14868383152041567No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT21040.1463878247631982No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGC20310.14130877951238383No Hit
CGTCAGATGTGTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACAT20040.13943022852920592No Hit
GTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG18130.1261412197222806No Hit
ATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCA17830.12405394085208288No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATG17820.12398436488974297No Hit
GGACTCCTCCTTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC17170.11946192733764797No Hit
GTGTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACATCTCCGAGC16970.11807040809084951No Hit
TCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTC16970.11807040809084951No Hit
CGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTC16820.11702676865575067No Hit
GTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCC16760.11660931288171113No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCTG15680.10909510894899943No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG15520.10798189355156065No Hit
GTATCAACGCAGAGTACATGGGCAACTTCTTAGAGGGACAAGTGGCGTTC14670.10206793675266719No Hit
GTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCA14660.10199836079032727No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCTGTCTCTT14640.10185920886564744No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)