FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004048713

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048713
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1019757
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACCGATCA189811.8613257864373571No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC81880.8029363858252505No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGA21930.21505123279369495No Hit
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGT20580.20181278481049897No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTC15780.1547427475369132No Hit
CGTCAGATGTGTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACAT15590.15287955856150043No Hit
GGACTCCTCCTTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC15490.15189893278496738No Hit
GTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCC14430.1415042995537172No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACCGATCA13170.12914841476940095No Hit
GTGTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACATCTCCGAGC12940.12689297548337497No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA12410.12169565886774986No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG11250.11032039985996664No Hit
GTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACA10720.10512308324434154No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)