FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004048717

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048717
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences345445
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAG150874.367410152122624No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC84772.453936227185225No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA13720.3971688691398052No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAG12950.37487877954522425No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCG12210.3534571349997829No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG11480.33232497213738804No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT9870.28571842116690066No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTC8800.2547438810809246No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA8540.24721735732171546No Hit
CGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTC6380.18468931378367034No Hit
GGACTCCTCCTTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC6040.17484693656008918No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTA5400.15632010884511283No Hit
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGCG4950.1432934331080201No Hit
TGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTC4830.13981965291146203No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA4820.13953017122841554No Hit
GTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCC4160.12042438014734619No Hit
GCGTAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGT4060.11752956331688112No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC3990.11550319153555558No Hit
TCCTCAATGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGTAGT3800.11000303955767199No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCC3670.10623977767806742No Hit
GTGTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACATCTCCGAGC3540.10247651579846286No Hit
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG3540.10247651579846286No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)