FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004048720

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048720
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences709555
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGC77261.0888514632410453No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT38440.5417479969840252No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC22850.3220328233893074No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCC15760.22211104142737348No Hit
GGACTCCTCCTTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC12740.17954915404725497No Hit
GTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCC11980.16883821550126488No Hit
GTGTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACATCTCCGAGC11180.1575635433475911No Hit
CGTCAGATGTGTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACAT8510.11993432503470484No Hit
ATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTT8500.11979339163278394RNA PCR Primer, Index 47 (95% over 22bp)
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTC8140.11471978916363074No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)