FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004048722

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048722
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1285106
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACACTGAG340072.6462408548399896No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC211881.648735590682792No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACT37500.29180472272326174No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA25870.2013063513826875No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA24260.1887782019537688No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTC23900.18597687661562548No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACACTGAG23140.18006296756843404No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG21810.16971362673584903No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA16490.12831626340550897No Hit
CGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTC15700.12216891058013891No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC15200.11827818094382876No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGT15050.11711096205293571No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)