FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004048732

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048732
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1218143
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGC214821.7635039564320447No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC103020.8457135163933956No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATG27670.22714902930115757No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT27670.22714902930115757No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTC20220.16599036402130127No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA18830.15457955264693882No Hit
CGTCAGATGTGTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACAT16430.1348774322883274No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGC16370.1343848792793621No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA15860.1301981787031572No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG13240.10869003064500637No Hit
GGACTCCTCCTTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC12980.10655563427282347No Hit
CGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTC12950.10630935776834083No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12590.10335403971454911No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)