FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004048733

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048733
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1886985
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCGC631233.345177624623407No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC191811.0164892672702752No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAG53100.2814012829990699No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA53060.28118930463146236No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTC47240.25034645214455864No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG43690.23153337201938543No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCGC33750.1788567476689004No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA28810.15267741926936357No Hit
CGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTC24990.13243348516283912No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTA22120.11722403728699486No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA21260.11266650238343177No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20110.10657212431471369No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC19270.10212057859495438No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)