FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004048735

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048735
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1396864
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAG282712.023890657930908No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC215251.5409517318794097No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCT28290.20252508476129388No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA26070.18663234216072574No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGT23040.16494089617886923No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTC21820.15620704664162008No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG18740.13415765600659763No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA17520.1254238064693485No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACCCTAAG16680.11941033629616055No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA15730.11260938788600751No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14810.10602320626775406No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC13990.10015291395583249No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)