FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004048745

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048745
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1440013
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCGC936166.501052421054531No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC255751.7760256331019235No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAG118080.8199925972890522No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG103650.7197851686061167No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCGC95320.6619384686110473No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA91900.6381886830188339No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTC70930.4925649976771043No Hit
CGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTC38640.2683309109014988No Hit
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAG37830.2627059616822904No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTA33690.23395622122855836No Hit
TGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTC30180.2095814412786551No Hit
GCGTAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCGCT29840.207220351482938No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGT26600.18472055460610426No Hit
CGTCAGATGTGTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACAT19940.13847097213705709No Hit
GGACTCCTCCTTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC19160.13305435437041194No Hit
TCCTCAATGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGCGCT18570.1289571691366675No Hit
GTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCC17420.12097113012174196No Hit
AGGGCTTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGC15930.11062400131109928No Hit
GTGTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACATCTCCGAGC15670.1088184620555509No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)