FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004048758

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048758
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences902122
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACACTGAG169591.8799009446615869No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC72250.8008894584102816No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGT37080.4110308805239203No Hit
GGACTCCTCCTTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC22820.252959134130417No Hit
CGTCAGATGTGTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACAT22630.25085298884186397No Hit
GTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCC21240.2354448733098184No Hit
GTGTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACATCTCCGAGC19320.21416172092023034No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCC16080.1782464012628004No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACT15680.17381241118163618No Hit
GTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACA15430.17104116738090858No Hit
CGTCAGATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCT14470.16039959118611452No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTC12110.1342390497072458No Hit
GTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACA10880.12060453020766593No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATGTCGTATGCCGT10760.11927433318331666No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA10580.11727903764679277No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACACTGAG9190.10187092211474723No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)