FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004048766

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048766
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences745149
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGC60360.8100393344149961No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGT46660.6261834881345878No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC39570.5310347326507853No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCC20440.2743075545964633No Hit
GTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACA10990.14748728106727646No Hit
GGACTCCTCCTTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC10220.13715377729823164No Hit
GTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCC10100.13554336112643245No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9220.12373364253323832No Hit
ATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTT8870.11903659536549066Illumina PCR Primer Index 10 (95% over 22bp)
CGTCAGATGTGTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACAT8600.11541315897894246No Hit
CGTCAGATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCT8450.11340013876419347No Hit
GTGTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACATCTCCGAGC8410.11286333337359374No Hit
GTATCAACGCAGAGTACATGGGGTGGCGGCGACGACCCATTCGAACGTCT8110.10883729294409573No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGG7770.10427444712399801No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)