FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004048770

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048770
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1145417
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACACTGAG290942.5400356376760604No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC129611.1315529628074317No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACT42130.3678136434154548No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGT34840.30416870013279007No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACACTGAG25980.22681695836538132No Hit
CGTCAGATGTGTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACAT20910.18255360274904248No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTC20720.18089481821904163No Hit
GGACTCCTCCTTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC20290.17714072691430283No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG20270.176966118016408No Hit
GTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCC19350.16893410871324593No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA19090.16666419304061317No Hit
GTGTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACATCTCCGAGC18260.1594179237779778No Hit
GTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACA15950.1392505960711252No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCC15180.13252815350217434No Hit
CGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTC13990.12213892407743207No Hit
CGTCAGATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCT13810.12056744399637861No Hit
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACACT13620.11890865946637774No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11820.10319385865584325No Hit
GCGTAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTGAGC11660.10179698747268462No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)