FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004048772

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048772
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences739509
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACACTCGC97271.3153322001490177No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC43770.5918792063382596No Hit
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT40970.5540162459145189No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCC15270.2064883591680426No Hit
GGACTCCTCCTTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC13130.17755023941561224No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACT12890.17430484280786307No Hit
GTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCC11510.15564381231330518No Hit
CGTCAGATGTGTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACAT11000.14874734452183816No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTC10680.1444201490448392No Hit
GTGTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACATCTCCGAGC10520.14225655130633974No Hit
ATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT8120.1098025852288478Illumina PCR Primer Index 10 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7960.10763898749034832No Hit
GTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACA7880.10655718862109859No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)