FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004048782

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048782
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1441875
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACACTGAG460233.1918855656697014No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC226121.5682358040745557No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACT56030.38859124403987866No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACACTGAG33910.23517988729952322No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA32720.2269267446900737No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG30060.2084785435630689No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTC29280.20306892067620286No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA27340.1896142175986129No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17970.124629388816645No Hit
CGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTC17920.12428261811876896No Hit
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACACT17230.11949718248807975No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA16570.11491980927611617No Hit
GCGTAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTGAGC15130.10493281317728652No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)