FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004048788

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048788
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences913448
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCT353883.874112155262259No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC132141.446606703391983No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT57300.6272935076764085No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTC27530.3013855194822256No Hit
GGACTCCTCCTTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC26580.2909853653409937No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA25530.2794904581322637No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGA24300.2660249954020371No Hit
CGTCAGATGTGTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACAT23960.2623028349725436No Hit
GTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCC23860.2612080819050455No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCC23070.2525595326718105No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG22930.25102687837731325No Hit
GTGTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACATCTCCGAGC21620.23668561319308817No Hit
GTATCAACGCAGAGTACATGGGGTGGCGGCGACGACCCATTCGAACGTCT19160.209754687732635No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTA16660.18238586104518265No Hit
GTACATGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTT15900.17406573773219713No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCT14930.1634466329774656No Hit
GTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACA14560.15939604662772264No Hit
CGTCAGATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCT12440.13618728159676302No Hit
CGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTC12420.1359683309832634No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12120.13268407178076913No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT11900.1302756150322733No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGC10750.11768595475604522No Hit
ATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTT10460.11451117086030076TruSeq Adapter, Index 11 (95% over 23bp)
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT10100.1105700598173076No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCTGTCTCTTATACAC9660.10575314632031599No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA9410.10301626365157077No Hit
GTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACA9370.10257836242457152No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)