FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004048791

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048791
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences772457
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC274733.5565733756053737No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCT103761.3432462907320408No Hit
GTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACA36790.4762724656518097No Hit
CGTCAGATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCT33180.42953847269168377No Hit
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGT28440.368175833735729No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAC25700.3327046036219492No Hit
GTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACA23940.30992016384083515No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACCTGTCTCTTATACA20770.26888228082598775No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCC13920.18020420554153824No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACCTGTCTCTTATACACA11880.1537949685225197No Hit
GTGTATAAGAGACAGCCAGGTCTGAAGGCAGTCTCTTATACACATCTCCG11140.14421514725091494No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG10990.14227329158775182No Hit
GATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTAT10580.13696555277510591No Hit
GTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG10400.13463532597931016No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA9550.12363147722138579No Hit
GGACTCCTCCTTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC9510.12311364904454228No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCT9370.12130125042559003No Hit
CGTCAGATGTGTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACAT9040.11702916796663115No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCTGT8670.11223925733082876No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTC8540.1105563157560874No Hit
GTATAAGAGACAGGGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCC8470.10965011644661128No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)