FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004048813

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048813
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences420425
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCTGT72461.7234940833680206No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC69951.663792590830707No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCTG44111.0491764286139027No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCTGTCTCTT39860.9480882440387703No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCTGTC29130.6928703098055539No Hit
CGTCAGATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCT28210.670987691026937No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCCTGTCTCTTAT24240.5765594339061664No Hit
GTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACA19310.45929713979901293No Hit
GATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTAT14460.34393768210739134No Hit
GGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC12190.2899446988166736No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGCTGTCTC11370.2704406255574716No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGA9550.22715109710412085No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCCT9040.21502051495510496No Hit
GCGTCAGATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTC8380.19932211452696677No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA7910.18814295058571684No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC7180.17077956829398822No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA7030.1672117500148659No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACCTGTCTCTTATACA6720.1598382589046798No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGACTGTCT6470.1538918951061426No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCCTGTCTCT6090.14485342213236607No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGG5600.13319854908723316No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCTGTCTCTTATACAC4840.11512160313968008No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA4540.10798596658143544No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCTGTCTCTTATACACA4380.10418029375037165No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG4280.10180174823095677No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)