FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004048826

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048826
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences697720
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC7472410.709740296967265No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCA166032.3796078656194464No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACCTGTCTCTTATACA77371.108897552026601No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA64370.9225763916757439No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT54410.7798257180530872No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAG44060.6314854096199048No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG38700.5546637619675514No Hit
GTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACA37900.5431978444074987No Hit
CGTCAGATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCT37410.5361749699019664No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACCTGTCTCTTATACACA35710.5118098950868544No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCTGT35600.5102333314223471No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCTG29860.42796537292896863No Hit
GTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACA24680.3537235567276271No Hit
TCCTCAATGCTGTTGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGA23690.33953448374706185No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCTGTCTCTT23050.33036174969901966No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTA21940.31445278908444646No Hit
GGACTCCTCCTTGTCCCGCACTGTCTCTTATACACATCTCCGAGCCCACG21120.3027002235853924No Hit
GGACTCCTCCTTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC20620.2955340251103594No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA19440.27862179670928167No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCA19100.2737487817462592No Hit
GTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG18700.2680158229662329No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCC18470.2647193716677177No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCCTGTCTCTTAT17810.2552599896806742No Hit
ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCT15490.22200882875652123RNA PCR Primer, Index 1 (95% over 23bp)
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTC15050.21570257409849222No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCTGTC15030.2154159261594909No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT14070.2016568250874276TruSeq Adapter, Index 1 (95% over 21bp)
GTATCAACGCAGAGTACATGGGCAACTTCTTAGAGGGACAAGTGGCGTTC13800.1977870779109098No Hit
TTTGCAGATGATGGTGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC13410.1921974431003841No Hit
GCGTAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCAT13040.18689445622885972No Hit
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAG12660.18144814538783466No Hit
TGTCCATGGCATCCATAGCTGTCTCTTATACACATCTCCGAGCCCACGAG12640.18116149744883334No Hit
GTATCAACGCAGAGTACATGGGGTGGCGGCGACGACCCATTCGAACGTCT12520.17944160981482543No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCCT12200.17485524279080433No Hit
AGGGCTTCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGAC11840.1696955798887806No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA11350.1626727053832483No Hit
GATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTAT11000.15765636645072523No Hit
GTACATGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTT10350.14834030843318238No Hit
GCGTCAGATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTC10240.1467637447686751No Hit
TGGTGTCTGTGATGGTGCTGTCTCTTATACACATCTCCGAGCCCACGAGA9760.13988419423264345No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGG9530.1365877429341283No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT9320.13357793957461447No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC9020.12927822048959467No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC9000.12899157255059335No Hit
GTACATGGGCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGAT8540.12239866995356304No Hit
TGTCCATGGCATCCATCTGTCTCTTATACACATCTCCGAGCCCACGAGAC8500.12182537407556039No Hit
CTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAATAACA8420.12067878231955512No Hit
TGTCCATGGCATCCATAGCGACTGTCTCTTATACACATCTCCGAGCCCAC8310.11910221865504787No Hit
CGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTC8210.11766897896004128No Hit
TGTCCATGGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCA8020.11494582353952876No Hit
ATGCACTGCAGGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGC7780.11150604827151293No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGC7700.11035945651550765No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA7530.10792294903399645No Hit
GTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCA7420.10634638536948919No Hit
TGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCG7320.1049131456744826No Hit
AGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC7210.10333658200997536No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGCTGTCTC7140.10233331422347072No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCTGTCTCTTATACAC7100.10176001834546809No Hit
TGTCCATGGCATCCATAGCGCTGTCTCTTATACACATCTCCGAGCCCACG6980.10004013071146019No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)