FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004048827

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048827
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1022269
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC13332013.041577119134004No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCT259032.5338731781947805No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACCTGTCTCTTATACA133211.3030816741973004No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA108101.0574516100947988No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT92760.9073932595041032No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG68390.6690019945826392No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACCTGTCTCTTATACACA64760.6334927499513338No Hit
GTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACA63860.6246888050014233No Hit
CGTCAGATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCT58420.5714738488597424No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCTGT57130.558854861098204No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTC56360.5513225970855029No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCTG47780.46739165522969No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTA45610.44616436573935037No Hit
TCCTCAATGCTGTTGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGA43980.430219443218957No Hit
GTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACA38540.37700448707727613No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCTGTCTCTT38460.3762219141928396No Hit
GGACTCCTCCTTGTCCCGCACTGTCTCTTATACACATCTCCGAGCCCACG36920.3611573861674373No Hit
GGACTCCTCCTTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC33440.32711546569445027No Hit
GTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG32540.31831152074453983No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCC32030.31332261860625726No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCCTGTCTCTTAT31220.3053990681513379No Hit
ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCT26770.26186845145455845RNA PCR Primer, Index 39 (96% over 25bp)
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTC25090.24543442088139225No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCT24240.23711958398425462No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCTGTC23530.2301742496348808No Hit
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT23180.22675049326547123RNA PCR Primer, Index 39 (95% over 23bp)
TTTGCAGATGATGGTGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC23070.22567445554937107No Hit
TGTCCATGGCATCCATAGCTGTCTCTTATACACATCTCCGAGCCCACGAG21980.21501189999892398No Hit
TGGTGTCTGTGATGGTGCTGTCTCTTATACACATCTCCGAGCCCACGAGA19500.19075214058139298No Hit
GCGTAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTA19090.18674145454865598No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCCT18840.18429591428479197No Hit
GATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTAT17950.1755897909454361No Hit
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCTC17930.17539414772432696No Hit
TGTCCATGGCATCCATCTGTCTCTTATACACATCTCCGAGCCCACGAGAC16140.15788407943506064No Hit
CGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTC15910.15563418239230575No Hit
TGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCG15820.1547537878973147No Hit
GCGTCAGATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTC15800.15455814467620557No Hit
AGGGCTTCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGAC14690.14369994590464935No Hit
TGTCCATGGCATCCATAGCGACTGTCTCTTATACACATCTCCGAGCCCAC14060.13753718443971205No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA13670.1337221416280842No Hit
GTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCA13480.13186353102754755No Hit
TGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTC12870.12589641278371935No Hit
AGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC12640.12364651574096445No Hit
CATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCT12300.1203205809821094RNA PCR Primer, Index 39 (96% over 28bp)
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGCTGTCTC12280.12012493776100029No Hit
ATGCACTGCAGGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGC12250.1198314729293366No Hit
TGTCCATGGCATCCATAGCGCTGTCTCTTATACACATCTCCGAGCCCACG12060.11797286232879994No Hit
TGTCCATGGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCT11860.11601643011770874No Hit
TGTCCATGGCATCCATAGCGCCTGTCTCTTATACACATCTCCGAGCCCAC11410.11161445764275352No Hit
GTATCAACGCAGAGTACATGGGGTGGCGGCGACGACCCATTCGAACGTCT11340.11092970636887159No Hit
TTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTA11030.10789723644168021No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)