FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004048829

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048829
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences735729
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC729239.911665844353015No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGG204692.782138531986642No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACCTGTCTCTTATACA79751.083958903346205No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT65940.8962539195818026No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA63610.8645846500545716No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAG39640.5387853407980384No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG39340.5347077524468928No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCTGT36970.5024948044728426No Hit
CGTCAGATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCT36910.5016792868026134No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACCTGTCTCTTATACACA36890.5014074475792037No Hit
GTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACA36400.494747386605666No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCTG29980.40748699589115017No Hit
GGACTCCTCCTTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC28880.39253583860361624No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA27390.37228381645959313No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTA25580.3476823667410147No Hit
TCCTCAATGCTGTTGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGA24900.3384398331450847No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCTGTCTCTT23120.3142461422616208No Hit
GGACTCCTCCTTGTCCCGCACTGTCTCTTATACACATCTCCGAGCCCACG22050.2997027438092015No Hit
GTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACA21910.2977998692453335No Hit
GTATCAACGCAGAGTACATGGGCAACTTCTTAGAGGGACAAGTGGCGTTC21760.29576107506976074No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGG21260.28896509448451807No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCC20840.28325647079291427No Hit
GTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG17990.24451938145703106No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCCTGTCTCTTAT17930.24370386378680192No Hit
TGTCCATGGCATCCATAGCTGTCTCTTATACACATCTCCGAGCCCACGAG17800.24193690883463886No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA17240.23432541057916706No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT16490.22413143970130303RNA PCR Primer, Index 33 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCT16340.22209264552573027RNA PCR Primer, Index 33 (95% over 24bp)
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTC15360.20877252357865464No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCTGTC14840.2017047037700023No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGG14130.1920544113389577No Hit
GTACATGGGCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGAT13500.18349147580155192No Hit
TTTGCAGATGATGGTGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC13360.18158860123768397No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCCT13250.1800934855089306No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC13000.17669549521630926No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC12520.17017135385447632No Hit
GCGTAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGC12300.16718112239696953No Hit
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACAAG12050.16378313210434822No Hit
CTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAATAACA12020.16337537326923365No Hit
TGTCCATGGCATCCATCTGTCTCTTATACACATCTCCGAGCCCACGAGAC11990.16296761443411908No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA10740.14597766297101242No Hit
GCGTCAGATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTC10250.13931760199747462No Hit
GATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTAT10030.13632737053996785No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGA9840.13374489791757563No Hit
TGGTGTCTGTGATGGTGCTGTCTCTTATACACATCTCCGAGCCCACGAGA9830.13360897830587076No Hit
GGTATCAACGCAGAGTACATGGGCAACTTCTTAGAGGGACAAGTGGCGTT9740.1323857018005271No Hit
TCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAATAACAGG9610.130618746848364No Hit
GTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTGCCTACCC9410.12790035461426694No Hit
AGGGCTTCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGAC9310.12654115849721842No Hit
GAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCG9160.1245023643216456No Hit
GGACTCCTCCTTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC9120.1239586858748262No Hit
ACGCAGAGTACATGGGCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCAC9000.12232765053436796No Hit
CGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTC8970.1219198916992534No Hit
TTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGC8940.12151213286413884No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCTGTCTCTTATACAC8760.11906557985345147No Hit
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG8410.1143083934437816No Hit
TGTCCATGGCATCCATAGCGACTGTCTCTTATACACATCTCCGAGCCCAC8380.11390063460866705No Hit
TGTCCATGGCATCCATAGCGCTGTCTCTTATACACATCTCCGAGCCCACG8160.11091040315116028No Hit
TGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCG8150.11077448353945542No Hit
TGTCCATGGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGG8060.10955120703411174No Hit
GTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCA7890.10724057363512925No Hit
GAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTT7800.10601729712978555No Hit
GGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGAGCCAGTCAG7750.10533769907126128No Hit
ACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCGCGGGTAA7720.10492994023614674No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGCTGTCTC7650.10397850295421275No Hit
GTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC7370.10017275382647686No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)