FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004048844

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048844
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1066030
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC13501112.66484057671923No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTCAT131771.2360815361668995No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACCTGTCTCTTATACA115061.0793317261240303No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA83830.7863756179469621No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG74090.6950085832481262No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACCTGTCTCTTATACACA57690.5411667589092239No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGT54180.5082408562610808No Hit
CGTCAGATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCT49340.46283875688301457No Hit
GTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACA45570.42747389848315714No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCTGT44230.41490389576278347No Hit
TCCTCAATGCTGTTGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGA37770.354305225931728No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCTG37430.3511158222564093No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCT34640.32494395092070577No Hit
GGACTCCTCCTTGTCCCGCACTGTCTCTTATACACATCTCCGAGCCCACG33020.30974738046771666No Hit
GTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG33010.3096535744772661No Hit
GTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACA30300.284232151065167No Hit
TGTCCATGGCATCCATAGCTGTCTCTTATACACATCTCCGAGCCCACGAG27910.2618125193474855No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCTGTCTCTT27260.2557151299681998No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTA26390.24755400879900188No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA21500.20168287946868285No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCCTGTCTCTTAT21140.19830586381246307No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCC20820.19530407211804546No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCTGTC18920.17748093393244094No Hit
TTTGCAGATGATGGTGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC18320.17185257450540792No Hit
TGTCCATGGCATCCATCTGTCTCTTATACACATCTCCGAGCCCACGAGAC18190.17063309662955076No Hit
TGGTGTCTGTGATGGTGCTGTCTCTTATACACATCTCCGAGCCCACGAGA16880.1583445118805287No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTCAT16290.15280995844394624No Hit
GGACTCCTCCTTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC15690.14718159901691322No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCCT15230.14286652345618792No Hit
TGTCCATGGCATCCATAGCGACTGTCTCTTATACACATCTCCGAGCCCAC15020.14089659765672635No Hit
GATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTAT14050.131797416583023No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA13600.12757614701274822No Hit
TGTCCATGGCATCCATAGCGCTGTCTCTTATACACATCTCCGAGCCCACG13450.12616905715599No Hit
GCGTCAGATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTC13070.12260442951886907No Hit
GTATCAACGCAGAGTACATGGGCAACTTCTTAGAGGGACAAGTGGCGTTC13000.12194778758571523No Hit
TGTCCATGGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTCAT12960.12157256362391301No Hit
GCGTAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTCATG11960.11219196457885801No Hit
GTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCA11520.10806450099903381No Hit
TGTCCATGGCATCCATAGCGGCTGTCTCTTATACACATCTCCGAGCCCAC11320.1061883811900228No Hit
TGTCCATGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTCATG10970.10290517152425353No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGG10910.10234233558155023No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC10840.1016856936483964No Hit
TGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCG10700.1003724097820887No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)