FastQCFastQC Report
Sun 11 Sep 2022
EGAF00004048855

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048855
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3327998
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC34261010.294777821380901No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAG311010.9345258019986791No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA221650.6660160252500152No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG182770.5491890319645625No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTA101060.3036660478762307No Hit
TCCTCAATGCTGTTGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGA99790.29984993981366576No Hit
GGACTCCTCCTTGTCCCGCACTGTCTCTTATACACATCTCCGAGCCCACG86240.2591347711146461No Hit
GTATAATAACTGGCCTCCGATGTACACTTTTGGCCAGGGGACCAAGGTGG86120.2587741939748762No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC76740.2305890808828611No Hit
GTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG71920.21610589910210282No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA64920.19507223261552442No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTC64830.19480179976069698No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT62310.18722967982552874No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA61300.18419482223246528No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC60490.18176092653901835No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACCTGTCTCTTATACA58840.176802990867182No Hit
GTTATTATACTGCTGACAGTAATAAAATGCAAAATCTTCAGACTGCAGGC57410.17250611328492385No Hit
GTCCCAGACCCACTGCCACTGAACCTGACTGGGATACCTGTGGCCCGGGT57270.17208543995519227No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC50730.15243398583773188No Hit
CTCCTGCAGGGCCAGTCAGAGTATTGGCGGCAACTTAGCCTGGTACCAGC50680.15228374536282774No Hit
TTTGCAGATGATGGTGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC49080.14747605016589554No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC47700.1433294130585415No Hit
GTAGTAGACGGCCGTGTCGTCAGATGACAGGCCTCTCAGCTCCATGAAGG47150.14167676783459607No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG46170.13873205452647508No Hit
TGGTGTCTGTGATGGTGCTGTCTCTTATACACATCTCCGAGCCCACGAGA43780.1315505598260576No Hit
GTCTGTGTCTCCAGGGCAAAGAGCCACTCTCTCCTGCAGGGCCAGTCAGA43660.13118998268628765No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT43260.12998805888705464No Hit
GTATCAACGCAGAGTACATGGGGGAGGAACTGCTCAGTTAGGACCCAGAC42620.12806498080828171No Hit
CCTGTGGCCCGGGTGGAGGCATCATAGATGAGGAGCCTGGGAGGCTGGCC41730.1253907003549882No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT41520.12475969036039083No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT41370.12430896893567844No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC38870.11679694519047187No Hit
CTTTTGGCCAGGGGACCAAGGTGGAGATCAAAGGAACTGTGGCTGCACCA38580.11592555043602791No Hit
GAGTATTGGCGGCAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGCCTC38270.1149940594916223No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG38070.11439309759200576No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGC37650.11313107760281106No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA37600.11298083712790692No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTG36970.11108780714411486No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAG36380.10931496954024611No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATT36350.10922482525530364No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC36310.10910463287538033No Hit
GAGTGAACTCTGTCCCAGACCCACTGCCACTGAACCTGACTGGGATACCT36250.10892434430549537No Hit
TGTCCATGGCATCCATAGCGACTGTCTCTTATACACATCTCCGAGCCCAC36240.10889429621051454No Hit
GTCTACTACTGTGCGAGAGAAGAGCGGCTAAGTACTGGCTGGTCGTCCCT36240.10889429621051454No Hit
CTGCAGGAGAGAGTGGCTCTTTGCCCTGGAGACACAGACAGGGTGGCTGG35800.10757218003135818No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT35300.10606977528231688No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTC35200.1057692943325086No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCCTTTGATCTCCACCTTGGTCC34710.10429693767844811No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA34610.10399645672863986No Hit
GTGCTGACACAGTCGCCAGCCACCCTGTCTGTGTCTCCAGGGCAAAGAGC34530.10375607196879326No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG34530.10375607196879326No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG34310.10309501387921506No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG34240.1028846772143493No Hit
GGCCACAGGTATCCCAGTCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGT34190.10273443673944516No Hit
CTGCTACTCTGGCTGCCAGATAGCATTGGAGAAATAGTGCTGACACAGTC33750.1014123205602888No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT33720.10132217627534632No Hit
GCGTAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGG33680.10120198389542301No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)