FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004048863

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048863
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1804155
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC30775017.05784702533873No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCT326051.8072172291183408No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA192921.0693094551188784No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG191311.0603856098838513No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACCTGTCTCTTATACA113640.6298793618064967No Hit
TCCTCAATGCTGTTGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGA99550.5517818590974722No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTC84150.46642333945808423No Hit
GGACTCCTCCTTGTCCCGCACTGTCTCTTATACACATCTCCGAGCCCACG76210.4224138169946596No Hit
GTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG60570.33572503471154086No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACCTGTCTCTTATACACA56250.31178030712438787No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT55830.3094523474978591No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTA43500.2411101041761933No Hit
GTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACA43410.24061125568479425No Hit
TGGTGTCTGTGATGGTGCTGTCTCTTATACACATCTCCGAGCCCACGAGA43370.24038954524417247No Hit
TTTGCAGATGATGGTGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC43060.23867128932935364No Hit
CGTCAGATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCT42320.23456964617785056No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA39720.2201584675374344No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCT38820.2151699826234442No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCTGT38370.2126757401664491No Hit
TGTCCATGGCATCCATAGCGACTGTCTCTTATACACATCTCCGAGCCCAC37530.2080198209133916No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCTG35050.1942737735948408No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTC29670.16445371933121045No Hit
GCGTAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTA27560.1527584935884112No Hit
GTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACA26660.147770008674421No Hit
TGTCCATGGCATCCATAGCGGCTGTCTCTTATACACATCTCCGAGCCCAC26610.14749287062364375No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCTGTCTCTT25830.1431695170315189No Hit
AGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC25820.14311408942136344No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA25700.1424489580994981No Hit
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCTC24720.13701705230426434No Hit
CGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTC23590.13075373235669885No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC22190.12299386693493629No Hit
AGGGCTTCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGAC21460.1189476513935887No Hit
TTGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA20450.11334946276788857No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCC20350.1127951866663341No Hit
GTCCTCAATGCTGTTGGGCTGTCTCTTATACACATCTCCGAGCCCACGAG19400.10752956370156666No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGG18960.10509074885472701No Hit
TGTCCATGGCATCCATAGCTGTCTCTTATACACATCTCCGAGCCCACGAG18640.10331706532975271No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCCTGTCTCTTAT18570.10292907205866458No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA18400.10198680268602199No Hit
ATGCACTGCAGGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGC18170.10071196765244672No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)