FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004048864

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048864
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences804662
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC607957.555346219903512No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT107031.3301237041142742No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACCTGTCTCTTATACA88271.0969823354402222No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGC45650.5673189488257182No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACCTGTCTCTTATACACA44020.5470619962170451No Hit
GTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACA42350.526307940476871No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA39990.4969788557183016No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCC39740.4938719611464192No Hit
CGTCAGATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCT39210.4872853446540286No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCTGT30960.3847578237819109No Hit
TGTCCATGGCATCCATAGCTGTCTCTTATACACATCTCCGAGCCCACGAG29210.3630095617787344No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG27340.3397699903810544No Hit
GTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACA26650.33119496136265913No Hit
GTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG24590.3055941500903485No Hit
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT24170.30037456720958616TruSeq Adapter, Index 10 (95% over 21bp)
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCTG23990.2981376031178309No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTA22740.28260313025841904No Hit
ACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCT21850.27154258558251787RNA PCR Primer, Index 10 (95% over 23bp)
TGTCCATGGCATCCATCTGTCTCTTATACACATCTCCGAGCCCACGAGAC20090.2496700477964661No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGA18190.22605764905016018No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCTGTCTCTT17280.2147485528085084No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCCTGTCTCTTAT13950.17336471711103543No Hit
GGACTCCTCCTTGTCCCGCACTGTCTCTTATACACATCTCCGAGCCCACG13910.17286761397953426No Hit
TGTCCATGGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGC12730.15820307160024955No Hit
TGTCCATGGCATCCATAGCGCTGTCTCTTATACACATCTCCGAGCCCACG12570.15621465907424484No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCTGTC11700.14540266596409424No Hit
GATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTAT11630.1445327354839672No Hit
TCCTCAATGCTGTTGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGA11290.14030735886620715No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCCT10960.13620625803132247No Hit
GTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCA10610.13185660563068716No Hit
GGACTCCTCCTTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC10190.1266370227499248No Hit
TGTCCATGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCT10110.12564281648692247No Hit
TGTCCATGGCATCCATAGCGTCTGTCCTGTCTCTTATACACATCTCCGAG9900.12303302504654129No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCCTGTCTCTTATACACATCTCCG9120.11333951398226833No Hit
TGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCG9010.1119724803706401No Hit
GCGTCAGATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTC8980.1115996530220142No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)