FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004048878

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048878
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2497162
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC39510715.82224140844687No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGC344611.380006583473559No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA274721.1001288662890112No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG222220.8898902033588529No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACCTGTCTCTTATACA139430.5583538432828947No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAA125630.5030911090269674No Hit
TCCTCAATGCTGTTGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGA101310.40570055126579696No Hit
GTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG94200.3772282294861126No Hit
GGACTCCTCCTTGTCCCGCACTGTCTCTTATACACATCTCCGAGCCCACG91340.36577522803886975No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACCTGTCTCTTATACACA67210.26914553401020835No Hit
TTTGCAGATGATGGTGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC59350.23766980276009325No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGC53800.2154445726789051No Hit
CGTCAGATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCT51970.20811625357105384No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCTGT50700.20303048020112432No Hit
GTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACA49060.19646302482578226No Hit
TGGTGTCTGTGATGGTGCTGTCTCTTATACACATCTCCGAGCCCACGAGA48610.19466097914352373No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA48400.19382002449180308No Hit
TGTCCATGGCATCCATAGCGACTGTCTCTTATACACATCTCCGAGCCCAC46290.18537043251499102No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCTG44740.17916338627610062No Hit
GCGTAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCG40040.16034202026140074No Hit
TGTCCATGGCATCCATAGCGGCTGTCTCTTATACACATCTCCGAGCCCAC38550.15437524678014483No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT36350.14556524566688103No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTA35470.14204124522157552No Hit
GTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACA32680.13086856199157282No Hit
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAA32670.1308285165319671No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCTGTCTCTT31430.1258628795408548No Hit
TTGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA31180.12486174305071118No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA30720.12301965190884692No Hit
AGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC28460.11396937803794867No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTC27650.11072569580988338No Hit
AGGGCTTCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGAC26140.10467883140941596No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC25030.10023378539317834No Hit
CGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTC25000.1001136490143611No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)