FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004048882

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048882
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1483531
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC28862419.455205182770026No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACT283111.9083524375291112No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA186791.2590906425278607No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG183821.2390708384253515No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACCTGTCTCTTATACA140610.9478062810955753No Hit
TCCTCAATGCTGTTGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGA99600.6713712082861767No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGT88430.5960778709713515No Hit
GTATCAACGCAGAGTACATGGGGTGGCGGCGACGACCCATTCGAACGTCT84540.5698566460694114No Hit
GGACTCCTCCTTGTCCCGCACTGTCTCTTATACACATCTCCGAGCCCACG73520.4955744099718847No Hit
GTACATGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTT73240.4936870210329275No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACCTGTCTCTTATACACA65980.4447497221156821No Hit
GTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG64790.4367283191251143No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT64320.4335602019775791No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT51510.34721215802029076No Hit
GTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACA51280.34566180282043313No Hit
CGTCAGATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCT50790.3423588721772582No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCTGT49540.333933028699771No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA46620.314250258336361No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCTG43880.29578080943370916No Hit
TGGTGTCTGTGATGGTGCTGTCTCTTATACACATCTCCGAGCCCACGAGA42500.28647867823456336No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG41800.28176020588717055No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTA40580.27353658265314307No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACT39930.26915514404484975No Hit
GGTATCAACGCAGAGTACATGGGGTGGCGGCGACGACCCATTCGAACGTC38590.2601226398369835No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGC38160.25722414968072793No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT37350.2517642031073163No Hit
TGTCCATGGCATCCATAGCGACTGTCTCTTATACACATCTCCGAGCCCAC37210.2508205086378377No Hit
TTTGCAGATGATGGTGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC36910.24879830620324078No Hit
GTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACA33550.22614963893575532No Hit
ACGCAGAGTACATGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTA31420.21179200165011716No Hit
GTATCAACGCAGAGTACATGGGCAACTTCTTAGAGGGACAAGTGGCGTTC30860.20801722377220294No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCTGTCTCTT29540.19911953305997648No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA28920.19494031469514286No Hit
ATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCA27120.18280710008756137No Hit
CGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGAC26980.18186340561808279No Hit
TGTCCATGGCATCCATAGCGGCTGTCTCTTATACACATCTCCGAGCCCAC26950.1816611853746231No Hit
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCGT26520.17876269521836752No Hit
GGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCA26380.17781900074888898No Hit
GCGTAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTA25960.1749879173404533No Hit
ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGC25580.1724264609232972No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTC24970.16831464930628345No Hit
GGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTT24590.16575319288912738No Hit
AGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC23510.15847326412457846No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCCTGTCTCTTAT23150.15604662120306215No Hit
CGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCT23140.15597921445524224No Hit
ATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGAC22450.15132814885566934No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCC22430.15119333536002955No Hit
ATGCACTGCAGGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGC21860.14735115073429542No Hit
TTGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA21690.14620523602135715No Hit
GTCCTCAATGCTGTTGGGCTGTCTCTTATACACATCTCCGAGCCCACGAG21580.1454637617953383No Hit
AGGGCTTCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGAGAC21430.14445266057803982No Hit
GAGTACATGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAAC21330.14377859309984087No Hit
GTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTT20940.14114972993486485No Hit
GTACATGGGCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGAT20900.14088010294358527No Hit
CTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAG20840.14047566245666587No Hit
TGTCCATGGCATCCATAGCTGTCTCTTATACACATCTCCGAGCCCACGAG20710.13959937473500722No Hit
ATCAACGCAGAGTACATGGGGTGGCGGCGACGACCCATTCGAACGTCTGC20350.1371727318134909No Hit
GCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGA20150.13582459685709297No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCG20060.1352179361267139No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGG19830.13366758092685627No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC19710.1328586999530175No Hit
GCCTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAG19590.13204981897917872No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCTGTC19570.13191500548353893No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC19350.13043205703150118No Hit
CTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAATAACA18740.12632024541448747No Hit
CCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGCACGGCGACTACCA18490.12463507671899005No Hit
CGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTC18330.12355656875387168No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCCT17880.12052326510197629No Hit
GAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATC17590.11856846941519929No Hit
GGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCA17530.1181640289282799No Hit
GGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATT17360.11701811421534164No Hit
ATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGCACGGCGA17210.11600701299804318No Hit
TATCAACGCAGAGTACATGGGGTGGCGGCGACGACCCATTCGAACGTCTG17170.1157373860067636No Hit
CTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT16920.11405221731126615No Hit
TGTCCATGGCATCCATAGCGCCTGTCTCTTATACACATCTCCGAGCCCAC16550.11155816764192997No Hit
GTGCCTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGG16300.10987299894643253No Hit
TCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCC16250.10953596520733304No Hit
ACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCT15960.10758116952055603No Hit
CCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGG15500.10448045912084074No Hit
TGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCG15470.10427823887738105No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA15410.10387379839046167No Hit
GGACTCCTCCTTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC15370.10360417139918209No Hit
TTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCA15250.10279529042534331No Hit
GTATCAACGCAGAGTACATGGGGGCGGCGACGACCCATTCGAACGTCTGC15190.10239084993842393No Hit
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG14980.10097530823420608No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)