FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004048891

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048891
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences786610
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC506516.439150277774247No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACCTGTCTCTTATACA76200.968713848031426No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAG60600.7703944775682994No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT41010.5213511142751809No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA38020.4833399016030816No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACCTGTCTCTTATACACA35380.4497781619862448No Hit
GTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACA32210.4094786488857248No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCTGT27730.3525253937783654No Hit
CGTCAGATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCT25900.32926100608942166No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCTG22650.2879444705762703No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG22210.2823508473067975No Hit
GTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACA18840.239508778174699No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA18450.23455079391312086No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTA17700.2250162087947013No Hit
GTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG17190.21853269091417601No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTA17040.21662577389049212No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCTGTCTCTT16520.2100151282083879No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCCTGTCTCTTAT15590.19819224266154767No Hit
TGTCCATGGCATCCATAGCTGTCTCTTATACACATCTCCGAGCCCACGAG15270.19412415301102198No Hit
GGACTCCTCCTTGTCCCGCACTGTCTCTTATACACATCTCCGAGCCCACG14540.18484382349576028No Hit
TCCTCAATGCTGTTGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGA13360.16984274290944687No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCC12960.16475763084628978No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA11660.14823101664102922No Hit
GGACTCCTCCTTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC11490.14606984401418746No Hit
GTATCAACGCAGAGTACATGGGCAACTTCTTAGAGGGACAAGTGGCGTTC11480.14594271621260851No Hit
ACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCT10290.13081450782471618RNA PCR Primer, Index 3 (95% over 23bp)
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT10160.12916184640419012TruSeq Adapter, Index 3 (95% over 21bp)
TGTCCATGGCATCCATCTGTCTCTTATACACATCTCCGAGCCCACGAGAC10150.1290347186026112No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCTGTC9950.12649216257103266No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCCT9230.1173389608573499No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGG9070.11530491603208705No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC8730.11098257077840352No Hit
TTTGCAGATGATGGTGCTGTCTCTTATACACATCTCCGAGCCCACGAGAC8720.1108554429768246No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA8060.10246500807261541No Hit
TGTCCATGGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAG8030.10208362466787861No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)