FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004048892

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048892
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50918
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCTGT19793.8866412663498178No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCTGTC12852.5236655013943987No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGCTGTCTC6351.2471031855139636No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCTGTCTCTT5761.1312306060725088No Hit
CGTCAGATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCT5571.0939157076083112No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCTG5421.0644565772418397No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC5361.0526729250952513No Hit
GATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTAT3740.7345143171373582No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCCTGTCTCTTAT2160.42421147727719083No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1850.36332927451981617No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC1710.33583408617777605No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGG1700.3338701441533446No Hit
ATGCCCTGCAGTGCATCTGTCTCTTATACCTGTCTCTTATACACATCTCC1350.26513217329824423No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCTGTCT1270.24942063710279272No Hit
GCGTCAGATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTC1200.23567304293177263No Hit
GTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTCTTATACACA1110.2179975647118897No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCTGTCTCTTATACACA1080.21210573863859541No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCTGTCTCTTATA1070.21014179661416393No Hit
GTCAGATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCTGTCTC1070.21014179661416393No Hit
GGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGAGCCAGTCAG1070.21014179661416393No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA990.19443026041871245No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTGTCTCTTATA960.18853843434541812No Hit
ATGCCCTGCAGTGCATCTGTCTCTTATACACCTGTCTCTTATACACATCT890.17479084017439805No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCTGTCTCTTAT830.16300718802780942No Hit
CTAGTTACGCGACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGA820.16104324600337797No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT810.15907930397894654No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC800.15711536195451511No Hit
CATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTCTGCT800.15711536195451511TruSeq Adapter, Index 8 (96% over 27bp)
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCTGTCTC740.14533170980792648No Hit
AGGAAGCCCTCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC730.14336776778349503No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCTGTCTCTTAT680.13354805766133784No Hit
CGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCA670.13158411563690642No Hit
ATGCACTGCAGGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGC640.1256922895636121No Hit
GTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACCAG630.12372834753918065No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACCTGTCTCTTATACA620.1217644055147492No Hit
TCCTCAATGCTGTTGGGCTGTCTCTTATACACATCTCCTGTCTCTTATAC620.1217644055147492No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCCTGTCTCT620.1217644055147492No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA620.1217644055147492No Hit
GGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGAGCCAGTCAGT610.11980046349031777No Hit
TCCTCAATGCTGTTGGGCTGTCTCTTATACACATCTCCGAGCCTGTCTCT610.11980046349031777No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC600.11783652146588632No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCTGTC600.11783652146588632No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGCTGTCTCTTATACACATC590.11587257944145489No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGA590.11587257944145489No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCTGTCTCTTATACACAT570.11194469539259201No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCCT570.11194469539259201No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCCTGTCTCTTATACACATCT570.11194469539259201No Hit
GCAGCGTCAGATGTGTATAAGAGACAGTGTCCATGGCATCCATAGCGTCT560.10998075336816059No Hit
AAGTAAACGCTTCGGGCCCCGCGGGACACTCAGCTAAGAGCATCGAGGGG560.10998075336816059No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA540.10605286931929771No Hit
CGCGACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGG540.10605286931929771No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATC540.10605286931929771No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAT540.10605286931929771No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGA540.10605286931929771No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG530.10408892729486625No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCG520.10212498527043481No Hit
GAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTT520.10212498527043481No Hit
AACTAGTTACGCGACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGG510.10016104324600338No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)