FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004048909

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048909
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences643678
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGG238393.703559854461392No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC111071.7255522171023399No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT44030.6840376710094177No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAG30760.47787869089824414No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGG29390.4565947570058321No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG22960.3567000891750223No Hit
GGACTCCTCCTTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC21830.33914472764332476No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTC17030.2645732804290344No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA14470.2248018419147462No Hit
CGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTC14120.21936434055537085No Hit
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACAAG13990.21734469719331714No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACCTGTCTCTTATACA12900.20041076438840538No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCC12010.18658397521742237No Hit
ACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCT11610.18036968794956484RNA PCR Primer, Index 33 (95% over 24bp)
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA11060.17182504295626075No Hit
TGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTC10540.16374646950804594No Hit
GCGTAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGC10080.15660003915000978No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT9930.1542696814245632RNA PCR Primer, Index 33 (95% over 22bp)
CTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTA9730.15116253779063443No Hit
GTATCAACGCAGAGTACATGGGGCAGGCCTCTGCAGTCGACGGGCCCGGT8670.134694676530812No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA8140.12646074590090076No Hit
GTGTATAAGAGACAGCCAGGTCTGAAGGCAGTCTCTTATACACATCTCCG7790.12102324454152542No Hit
ATGCCCTGCAGTGCATCTGTCTCTTATACCTGTCTCTTATACACATCTCC7490.11636252909063226No Hit
GGACTCCTCCTTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC7410.11511967163706077No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC7200.11185717082143556No Hit
CCTTCAGACCTGGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCG6810.10579824073527447No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)