FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004048912

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048912
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2114309
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC723743.423056894711227No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACT655823.101817189445819No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGT275051.30089783470628No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG157230.7436472152367511No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACT95460.45149502745341386No Hit
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCGT71040.3359962995002149No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA65310.30889524662667567No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA52990.2506256181097465No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTC50860.2405514047379073No Hit
GCGTAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTA45750.2163827520007719No Hit
CGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTC36170.1710724402156922No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA31130.1472348649133121No Hit
TGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTC27940.13214719324375007No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC23360.11048526965547609No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21760.10291778543249827No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC21750.10287048865610467No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)