FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004048914

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048914
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences807204
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTC590807.319091580319226No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC320713.973097259181074No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGA158951.9691428684694325No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG105211.303387991139786No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTC83991.040505250221753No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA59910.7421915649575571No Hit
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGGA53770.6661265305920189No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTC38820.48091932150980427No Hit
GTATCAACGCAGAGTACATGGGCACTCTAGAGACTGTCAGGGAACGGTGC28930.35839762934772373No Hit
GCGTAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCC26050.3227189161599794No Hit
CTCTAGAGACTGTCAGGGAATCAATGCACCGTTCCCTGACAGTCTCTAGA23530.2915000421207031No Hit
CGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTC17530.21716938964623564No Hit
TGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTC15050.18644605329012245No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTA14700.18211009856244517No Hit
GGACTCCTCCTTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC14460.1791368724634665No Hit
GGTATCAACGCAGAGTACATGGGCACTCTAGAGACTGTCAGGGAACGGTG14420.17864133478030336No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA13960.17294265142392753No Hit
TCCTCAATGCTGTTGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGA12700.15733321440428938No Hit
GTATCAACGCAGAGTACATGGGGCAGGCCTCTGCAGTCGACGGGCCCGGT11570.14333427485493133No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT11020.1365206317114385No Hit
AGGGCTTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACGGAC10900.13503401866194917No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10740.13305186792929669No Hit
AGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTC10450.1294592197263641No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9980.12363665194919748No Hit
ACTCTAGAGACTGTCAGGGAATCAATGCACCGTTCCCTGACAGTCTCTAG9850.12202615447891735No Hit
TCCTCAATGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCC9410.11657523996412306No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA9160.1134781294443536No Hit
GTATCAACGCAGAGTACATGGGCTAGAAAAAAATGCCAGTTCACCGTTTG8300.10282406925634659No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)