FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004048919

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048919
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1614124
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC338792.09890937747038No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAG337932.093581410102322No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTA115620.7163018454592088No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG71090.44042465138985604No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA61200.3791530266571837No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAG43780.2712307108995344No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA35300.21869447452612067No Hit
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGTA29940.18548760813915163No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29660.18375292108908609No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA28150.17439800164051833No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC27600.17099058064931816No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC24690.15296222595042264No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGG23030.1426780098678912No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22230.1377217611534182No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA20630.12780926372447224No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTC20600.1276234043976795No Hit
GCGTAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGG19750.12235739013855193No Hit
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG19640.12167590594031188No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTT17650.10934723726306034No Hit
TTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGC17200.10655934736116927No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)