FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004048931

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048931
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1443838
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC518923.5940320174424No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACT283171.9612311076450406No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGT182791.266000756317537No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG129960.9001009808579633No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACT54080.37455725642350457No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA38890.26935154774981684No Hit
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCGT38660.2677585712524535No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA26680.18478527369414022No Hit
GCGTAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTA26640.1845082343032944No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACCTGTCTCTTATACA24370.16878624887279597No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT24030.16643141405060677No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTC21580.1494627513613023No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21020.14558419988946128No Hit
CGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTC18960.13131667126090324No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA17020.11788026080488254No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16720.11580246537353915No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)