FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004048933

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048933
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1406911
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC256311.8217925654145855No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGA162701.1564342023056184No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCC90600.643963974977806No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG49010.3483518147203341No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA36070.2563772690667711No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA24980.17755209817820744No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGA24120.17143941585501854No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA21620.15366999049691132No Hit
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCC18860.13405254490156093No Hit
GCGTAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAG18690.13284422397720966No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16700.11869976139215628No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC16320.115998808737724No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACCTGTCTCTTATACA15690.11152091354748098No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC15690.11152091354748098No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGG14630.10398667719564351No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)