FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004048952

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048952
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1373479
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC375352.73284120106678No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTCAT138351.007296070780842No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCT99650.7255298406455433No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG79920.5818800287445239No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26700.1943968564499348No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTCAT26380.19206700648499178No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA21780.15857541323893556No Hit
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGCT19730.14364981190101925No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19500.14197523223871644No Hit
GTATCAACGCAGAGTACATGGGCACTCTAGAGACTGTCAGGGAACGGTGC18780.1367330698175946No Hit
CTCTAGAGACTGTCAGGGAATCAATGCACCGTTCCCTGACAGTCTCTAGA15810.115109149830467No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACCTGTCTCTTATACA15410.11219683737428822No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)