FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004048973

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048973
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1378377
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACT337132.4458475438867597No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC310282.2510532314453884No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGT123460.8956910917695231No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG118400.858981251138114No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACT47200.3424317149807346No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA38750.2811277321081243No Hit
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCGT35020.2540669207335874No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA32810.2380335713669047No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTC24660.17890606125900244No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA22440.1628001628001628No Hit
GCGTAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTA21110.1531511335432904No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC16820.12202757300796517No Hit
TCCTCAATGCTGTTGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGA16480.11956090387462938No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGG15800.11462756560795775No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC15740.11419227105501616No Hit
CGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTC15500.11245109284324971No Hit
GGACTCCTCCTTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC14910.10817069640599053No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)