FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004048974

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048974
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1345287
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAG362542.69488964064917No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC208171.547402152849169No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGG104590.7774549222582244No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG65390.48606728527072657No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAG40720.3026863412788498No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA31400.2334074439134549No Hit
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACAGG30100.2237440784011144No Hit
GGACTCCTCCTTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC28630.21281704201408322No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTC23130.17193357253879657No Hit
GCGTAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGA19200.1427204752591826No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT17130.12733342402030198No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACCTGTCTCTTATACA14440.1073376907678436No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)