FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004048975

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048975
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1489292
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC1445399.705215632663037No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGA1067207.16582107471201No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG734584.932410836827163No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCC562163.7746795121440253No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGA229941.5439551142422037No Hit
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCC162031.0879666311240508No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA154261.0357941894537808No Hit
TCCTCAATGCTGTTGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGA95470.6410428579486092No Hit
GCGTAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAG82340.5528801605057974No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTC43370.29121220015953886No Hit
AGGGCTTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCT32880.22077604660469535No Hit
CGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTC27970.1878073608130575No Hit
TGTCCATGCATCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGA26790.17988413286313226No Hit
GTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG26150.1755867888902915No Hit
TGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTC23740.1594046029925629No Hit
AGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGA22430.15060847704815442No Hit
TCCTCAATGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAG20580.13818646712666152No Hit
GTCCTCAATGCTGTTGGGCTGTCTCTTATACACATCTCCGAGCCCACGAG20260.13603779514024114No Hit
TTGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA19120.12838315118861848No Hit
TGTCCATGGCATCCATAGCGACTGTCTCTTATACACATCTCCGAGCCCAC18990.1275102531941352No Hit
TCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCC16780.11267098728791937No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCGC16210.10884366531210804No Hit
GGACTCCTCCTTGTCCCGCACTGTCTCTTATACACATCTCCGAGCCCACG16140.10837364331507857No Hit
GTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACGA16010.10750074532059528No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)