FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004048976

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048976
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1055586
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC201731.9110711964728595No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTC128341.2158175648407614No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGA87850.8322391543654426No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG55410.5249217022582717No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTC27730.2626976864035711No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA21040.19932056696470019No Hit
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGGA19490.18463677994971514No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA17720.16786884251969997No Hit
GTATCAACGCAGAGTACATGGGCACTCTAGAGACTGTCAGGGAACGGTGC14160.1341434994401214No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA12790.12116492640107011No Hit
GCGTAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGACTCC12770.12097545818152192No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT12670.12002811708378094No Hit
CTCTAGAGACTGTCAGGGAATCAATGCACCGTTCCCTGACAGTCTCTAGA10940.10363911609286215No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)