FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004048978

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048978
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1622117
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC474002.9221073449079196No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGG456332.8131756217338206No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAG152140.9379101507474492No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG88340.5445969680362145No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGG60480.3728461017300232No Hit
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACAAG42660.2629896610417128No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA38770.23900865350649797No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTC32370.19955403956681297No Hit
GCGTAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGC26620.16410653485537727No Hit
CGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTC20710.1276726647954494No Hit
TGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTC17110.1054794444543766No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)