FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004048992

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004048992
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1874771
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAG810504.3231946728427095No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC582693.108059597678863No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGG108300.5776705528301856No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG88750.47339115017247435No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA69430.3703385640166186No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAG61430.32766668569121243No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTC48130.25672468797522474No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA35920.19159673368107358No Hit
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACAGG35320.18839634280666812No Hit
CGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTC29340.15649911375842704No Hit
GGACTCCTCCTTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC28630.15271198455704724No Hit
GCGTAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGA26260.14007044060314566No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA23570.12572202151622786No Hit
TGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTC21320.11372055573720737No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACCTGTCTCTTATACA19540.10422606280980451No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)