FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004049009

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004049009
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1946336
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC756793.8882803380300217No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTCAT596313.063756720319616No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCT123170.6328300971671901No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG91730.47129580915114344No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTCAT65910.3386362889038686No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA51470.2644456044588396No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTC36610.18809701921970307No Hit
CGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTC33090.17001175542146885No Hit
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGCT33020.16965210528911762No Hit
GCGTAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTCATG26310.13517707117373362No Hit
TGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTC23540.12094520165069134No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA22350.11483114940072012No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACCTGTCTCTTATACA20330.10445267415286981No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)