FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004049012

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004049012
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1681428
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGC256361.524656422992837No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC153070.9103571488044686No Hit
GGACTCCTCCTTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC48370.287672145343125No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGA35490.21107058999850128No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT33380.19852173271766616No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACCTGTCTCTTATACA26910.1600425352735889No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG20760.12346648206167615No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTC20330.12090913199970502No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA17710.10532713859885763No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCTGTCTCTTATACAC16930.10068822453295652No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)