FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004049045

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004049045
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1278994
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC386223.01971705887596No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACCGATCA237591.857631857538034No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG110980.8677132183575529No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGA100190.7833500391714112No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACCGATCA41850.327210291838742No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACCTGTCTCTTATACA25100.19624798865358245No Hit
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCGA25020.19562249705628018No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA20780.16247144239926067No Hit
GGTTAAGGGCCACGGCAAGAAGGTGGCCGACGCGCTGACCAACGCCGTGG19370.15144715299680842No Hit
GGACTCCTCCTTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC18890.1476942034129949No Hit
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGT17840.13948462619840282No Hit
GCGTAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGATCAG17300.1352625579166126No Hit
GGGTAAGGTCGGCGCGCACGCTGGCGAGTATGGTGCGGAGGCCCTGGAGA16140.12619292975572988No Hit
GACCAACGCCGTGGCGCACGTGGACGACATGCCCAACGCGCTGTCCGCCC16100.12588018395707876No Hit
CTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTG15390.12032894603102126No Hit
CCCGAAGCTTGTGCGCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGC14790.11563775905125434No Hit
CCCTTAACCTGGGCAGAGCCGTGGCTCAGGTCGAAGTGCGGGAAGTAGGT14690.11485589455462653No Hit
GCGTTGGTCAGCGCGTCGGCCACCTTCTTGCCGTGGCCCTTAACCTGGGC14680.11477770810496375No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTC14560.11383947070901035No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACCTGTCTCTTATACACA13390.10469165609846488No Hit
GTATCAACGCAGAGTACATGGGGACTCTTCTGGTCCCCACAGACTCAGAG12910.10093870651465137No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)