FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004049046

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004049046
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences482234
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACTGCAGC77661.6104214966178243No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC75581.567288909533546No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT38660.801685488787601No Hit
GGACTCCTCCTTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC21000.43547323498550494No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGC20480.42469008821443527No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG17110.35480700240961854No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCC13000.2695786692767411No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACCTGTCTCTTATACA11700.24262080234906702No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACTGCAGC10040.2081976799644986No Hit
ACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTCT8510.1764703442726975RNA PCR Primer, Index 29 (96% over 25bp)
ATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTT8420.1746040304084739RNA PCR Primer, Index 29 (95% over 23bp)
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTGC7270.1507566865878391No Hit
GGACTCCTCCTTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC7270.1507566865878391No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA6190.12836092021715598No Hit
CCTTCAGACCTGGCTGTCTCTTATACACCTGTCTCTTATACACATCTCCG6120.1269093427672043No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACCTGTCTCTTATACACA5820.12068829655312566No Hit
ATGCCCTGCAGTGCATCTGTCTCTTATACCTGTCTCTTATACACATCTCC5820.12068829655312566No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTC5550.11508935496045487No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCTGTCTCTTATACAC5540.11488198675331893No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTA4950.1026472625322976No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA4900.10161042149661782No Hit
GTATCAACGCAGAGTACATGGGGCAGGCCTCTGCAGTCGACGGGCCCGGT4900.10161042149661782No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)