FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004049049

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004049049
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1275685
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCT1049708.228520363569377No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC762145.974358873859926No Hit
CCTTCAGACCTGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTAC188671.47897012193449No Hit
TGTCCATGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCACG177081.3881169724500955No Hit
TGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCT93930.7363102960370311No Hit
ATGCACTGCAGGTACTTGACTGTCTCTTATACACATCTCCGAGCCCACGA82360.6456139250677088No Hit
CCTTCAGACCTGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTAC66560.5217588981605961No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTC39050.306110050678655No Hit
GCGTAACTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTG35510.27836025351085886No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA31180.24441770499770712No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA23030.1805304601057471No Hit
TCCTCAATGCTGTTGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGA22090.17316186989734927No Hit
CGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTC18600.1458040190172339No Hit
TCCTCAATGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTG18510.14509851569940857No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC17100.1340456303868118No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGG15170.11891650368233536No Hit
AGGGCTTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACTACG14810.11609449041103408No Hit
GGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC14410.11295892010958818No Hit
TGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTC14210.11139113495886523No Hit
GGACTCCTCCTTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC13540.10613905470394337No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA13170.10323865217510592No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)