FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004049050

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004049050
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences457084
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGC51061.1170813242204933No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT41570.909460843083547No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACATCTCCGAGCCCAC27670.605359189995712No Hit
GGACTCCTCCTTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC21570.47190450770536707No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCC14390.3148217833046005No Hit
ATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTT9660.2113397099876609RNA PCR Primer, Index 47 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTCT9390.20543269946005546RNA PCR Primer, Index 47 (95% over 24bp)
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA6970.1524883828792957No Hit
GGACTCCTCCTTGTCTCTTATACCTGTCTCTTATACACATCTCCGAGCCC6970.1524883828792957No Hit
GGACTCCTCCTTGTCTCTTATACACATCTCCGAGCCTGTCTCTTATACAC6320.13826780197950486No Hit
TGTCCATGGCATCCATAGCGTCTGTCTCTTATACACCTGTCTCTTATACA6230.13629879847030305No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA5820.12732889359505034No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC5420.11857776688748677No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTC5290.1157336507075286No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA5240.11463975986908315No Hit
GTATCAACGCAGAGTACATGGGGCAGGCCTCTGCAGTCGACGGGCCCGGT4740.10370085148462865No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGA4690.1026069606461832No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)